A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis
نویسندگان
چکیده
منابع مشابه
DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus)
We established polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and diagnostic PCR based on cytochrome C oxidase subunit I (COI) barcodes of Bungarus multicinctus, genuine Jinqian Baihua She (JBS), and adulterant snake species. The PCR-RFLP system utilizes the specific restriction sites of SpeI and BstEII in the COI sequence of B. multicinctus to allow its cleavage ...
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BACKGROUND The development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French Guiana. METHODS A descriptive study of the Leishmania species was identified, and their spatiotemporal distribution was conducted using patient...
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In this retrospective study, the usefulness of a PCR performed on serum for primary diagnosis and monitoring of Mediterranean visceral leishmaniasis (MVL) was assessed. In the case of primary diagnosis of MVL, the serum PCR showed a sensitivity of 97% and a specificity of 95%, with positive and negative predictive values of 94 and 97%, respectively.
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ژورنال
عنوان ژورنال: Asian Pacific Journal of Tropical Medicine
سال: 2020
ISSN: 1995-7645
DOI: 10.4103/1995-7645.275414